Effect of ERβ-regulated p38 signaling pathway on biological behaviors of prostate cancer cells
نویسندگان
چکیده
The study is aim to examine the effect of ERβ on the prostate cancer. We investigated several proliferation and apoptosis related genes in protein expression levels in vivo and in vitro. The anti-tumorigenic ability of ERβ was investigated to assess the effect of ERβ on the biological behaviors of LNCaP cells. Real-time RT-PCR and western blot showed that there was highest expression of p-p38 protein in pCDNA-3.1-ERβ group and lowest expression in pCDNA-3.1-ERβ+SB203580 group. There is no significant difference of p38 expression among the four groups. The expression of cyclin D1, Bcl-2 and MMP2 were highest in pCDNA-3.1-ERβ+SB203580 and lowest in pCDNA-3.1-ERβ group. Flow cytometry showed that ERβ could repress cell proliferation by increasing the percentage of cells in G0/ G1 phases. Soft colony forming experiments and transplantation tumor mode showed that ERβ could inhibit the tumor formation. Scratch experiments and transwell invasion experiment suggested that the number of migration cells was highest in pCDNA-3.1-ERβ+SB203580 group and lowest in pCDNA-3.1-ERβ group. In addition, immunohistochemical results showed that ERβ protein were positive expression in pCDNA-3.1-ERβ xenograft tumor and vascular endothelial growth factor (VEGF) was low expression compared to other groups. These results suggested that ERβ can inhibit malignant biological behavior of prostate cancer LNCaP cell by activating p38 signaling pathways and promote cell differentiation by inhibiting the expression of VEGF protein.
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